Vanquish

Vanquish UHPLC System: Best in UHPLC Column Thermostatting to Fit All Needs

The required technique to control the actual separation temperature is often underestimated and, in many cases, not well understood in UHPLC. Thermostatting has to be effective, but must not compromise the achievable column efficiency. On the other hand, it is very important to account for successful method transfer between different instruments. The Vanquish UHPLC system provides an entirely new and unique combination of active eluent pre-heating and advanced column thermostatting modes.

Vanquish UHPLC System: How Solvent Delivery Technology Can Improve Confidence in Peak Identification and Quantification

With the higher pressure capabilities of the Vanquish UHPLC system, the use of very long columns and column chains with sub-2 micron particles are no longer a challenge. Thus peak capacities beyond the common UHPLC range are now achievable, and even more complex matrices can be resolved. Additionally, this enhances requirements for reliable peak identification, with regards to higher retention time reproducibility, to maintain the level of data quality.

What Efficient Temperature Control Can Teach Us in Liquid Chromatography

Each good chromatographer wants to implement proper temperature control. For this purpose, column thermostats have evolved from simple units just providing a fixed temperature to high-tech devices. Elevated temperatures, well above the boiling point of the mobile phase, become more and more applied. Consequently, mobile phase pre-heating prior to entering the column as well as post-column mobile phase cooling prior to entering the detector becomes mandatory. In addition, viscous heating is a much discussed topic in Ultra High Performance Liquid Chromatography (UHPLC). When pressures increase to values far above 400 bar, friction between column and mobile phase generates high amounts of viscous heat and, thereby, an additional temperature increase inside the column.

UHPLC for Large Bio-Therapeutics

Seminar Slide Deck

Ken Cook EU Bio-separations Support Expert
June 2015

Why Have High Performance When You Can Have Ultra-High Performance?

Liquid chromatography plays an extremely important part in the development and production of biotherapeutic drugs. From separation of large quantities of antibodies in cell culture media to minute quantities of product from biological fluids during clinical studies, there is a growing need to be able to separate and measure these substances. The Russian botanist Mikhail Tsvet is considered to have invented the chromatographic technique (link to Lab Manager article) when he reported separations of different plant pigments into a series of colored bands on a packed column (see image below). He called this technique chromatography. HPLC became popular in the 1960s and 1970s, with UHPLC becoming popular in the 1990s. A clue as to the longevity of the technique is that the HPLC meeting (link to 2015 conference) is now in its 42nd year!

Antibodies Attack! What Happens When Biotherapeutics Go Bad?

It’s well known that biotherapeutic drugs (also known as biologics) are more difficult to produce consistently than our old friends, small molecule drugs. And, manufacturers spend untold amounts of money trying to make sure that each batch of biologics not only performs exactly the same way but also meets the strict regulatory requirements for consistency. By the way, here is a very informative 9-min video by Professor DeMasi at MCPHS University, Boston (USA), titled, Therapeutic Applications of Monoclonal Antibodies, that is worth a watch.

Antibodies as Therapeutics

Antibodies are an attractive choice as therapeutics for a number of reasons such as their antigen specificity, modes of action and their difficulty to be easily copied when coming off patent. Our own immune system has yielded us a powerful weapon which we can utilise to fight disease. However, as we produce these antibody therapeutics in vitro we bypass the immune systems careful in vivo control mechanisms to ensure antibody efficacy and safety. Therefore there are strict requirements to fully and comprehensively characterise antibody therapeutics to certify no changes to the antibody have occurred during its production which could affect its function. In this webinar, we shall look deeper in to antibodies themselves, how they develop, their structure, functions and modes of action. Why antibodies are such powerful biopharmaceuticals and how they are currently being utilised will also be discussed with relevant examples. This first section of the webinar will end with an overview of potential modifications that can occur to an antibody and the effects they have.

'Ultra' Biotherapeutic Characterisation

With the current market value for biopharmaceuticals standing at more than $150 billion (1), the need for higher throughput, more in-depth characterisation techniques for biologics is accentuated. Can recent technological advances in UHPLC meet the practical and regulatory requirements of the biotherapeutic development chain?

AppsLab Library - Biopharma Methods

The AppsLab Library of Analytical Applications is a fully searchable online, analytical method repository where you can find applications with detailed method information, chromatograms and related compound information. All the information needed to run, process and report the analysis is available in ready-to-use eWorkflows.

Fundamental UHPLC Workflows for Biotheraputic Characterization

The Medicine Maker - Piercing Together Protein Analysis

The Medicine Maker - Ultra-Engineering UHPLC from Scratch

Unlocking Biomolecular Secrets: Comprehensive Biopharmaceutical Characterization Workflows

BioLC Column Chemistries FlipBook

Pittcon 2016 – Day 3 – Adventures in Biopharma

Liquid Chromatography as the Future Replacement for Gel Electrophoresis

Five Instrument Requirements to Separate Your HPLC from the Status Quo

Challenging the Status Quo at HPLC 2016

Behavioural Economics, Black Swans and HPLC

Can LC/LC-MS Ever Replace Immunoassays?

LPG or HPG Pump for LC-MS – We Need Your Vote!

Biopharmaceutical Production in Your Garden Shed