Vanquish

Optimize Your Biotherapeutic Protein & Peptide Chromatography

The world of biotherapeutic characterization is undergoing a revolution. Development of evermore complex protein-based therapeutics places rigorous demands on analytical technologies.


Scientists require powerful and flexible solutions to fully characterize biotherapeutic proteins.

Because of their innovative architecture, the Thermo Scientific™ Vanquish™ Flex UHPLC systems were engineered to build your drug pipeline, catering specifically for the demanding analytical requirements that these molecules impose.

Vanquish Flex UHPLC systems have been designed and manufactured to exacting engineering standards to be truly

"Built for Biopharma."

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Vanquish Flex UHPLC delivers CONFIDENCE, with a fully biocompatible flow path and proven compliance. Achieve unprecedented PERFORMANCE in retention time stability, sensitivity and separation efficiency with the widest range of column chemistries for biotherapeutic proteins. Demand VERSATILITY through seamless integration with market-leading mass spectrometry, fluorescence and charged aerosol detection. Gain operational SIMPLICITY with easy, freely available, one-click workflows via AppsLab and tool-free Thermo Scientific™ Viper™ fittings.

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Protein Aggregate Analysis

Regulatory bodies typically impose a limit on the acceptable degree of protein aggregation that a biotherapeutic may exhibit, and therefore the confirmation of protein aggregate levels is vital throughout the drug development and production process. Our solutions for protein aggregation detection are rapid and reliable, giving you confidence in your ability to identify and separate higher order structures.

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Dimers, trimers and further higher order structures affect clinical efficacy and must be characterized, typically by size exclusion chromatography (SEC).

Our recommended SEC column, MAbPac SEC-1, uses spherical, fully porous ultrapure silica, typically with a 5 µm particle size and multiple column lengths.

Vanquish Flex UHPLC provides high sensitivity, high resolution SEC under non-denaturing conditions and in high-salt mobile phases.

Compliant, simple, data management and reporting is performed using Thermo Scientific™ Chromeleon™ CDS.

Baseline separation of trastuzumab monomer and oligomer with simultaneous detection of main compound and aggregates using MAbPac SEC.

Aggregation screening requires a powerful and flexible UHPLC. But column selection also plays a critical part in this workflow. MAbPac SEC has a proprietary hydrophilic bonded layer that results in minimal non-desired interactions between the stationary phase and the biomolecules. Its nonmetallic and biocompatible PEEK housing eliminates metal contamination from the column.


Resources
SEC-MS with Volatile Buffers for Characterization of Biopharmaceuticals HIC as a Complementary, Confirmatory Tool to SEC for the Analysis of mAb Aggregates 'Ultra' Biotherapeutic Characterisation AppsLab Library - Aggregate Methods Avoiding Deadly Aggregates in Bio-therapies: Part 1 of a Monoclonal Antibody Characterization Series
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Thermo Scientific™ MAbPac™ SEC-1 Size Exclusion LC Column Thermo Scientific™ MAbPac™ HIC-10 LC Column

Glycan Analysis

Even small changes in the type, composition or linkage of attached glycans can alter biotherapeutic efficacy, meaning that correct description is vital. We have a range of powerful and innovative chromatography solutions for released, labeled and non-labeled glycan analysis by HPLC and UHPLC.

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Multiple characterization techniques are possible; as free gycans, as glycopeptides, or as intact proteins.

Glycans can be labelled for easier detection, a typical example being fluorescence labelling for detection using a fluorescence detector (FLD).

GlycanPac columns have innovative mixed-mode surface chemistry, combining WAX and reversed phase or HILIC functionality for separation according to charge, size, and in the case of GlycanPac AXR-1, isomerism.

Vanquish Flex UHPLC system is fitted with a fluorescence detector, which is perfect for labelled glycans at the lowest concentrations. It also has a CAD detector, ideal for unlabelled glycans.

MS techniques for glycan proteins should ideally be high resolution accurate mass (HRAM) for maximum quantification and structural elucidation.

Six replicates of separation of N-glycans from fetuin using GlycanPac AXR-1 column.

Fetuin O-linked native glycans analyzed by UHPLC-CAD using GlycanPac AXH-1.

Detection of labelled glycans using FLD is well established, an example of this is shown for N-linked glycans in the upper of the two chromatograms (A). It is also desirable to separate and detect glycans without prior derivatization. The Vanquish Flex UHPLC Charged Aerosol Detector (CAD) has consistent analytical has consistent analytical response independent of chemical structure and a dynamic range of four orders of magnitude. CAD data of unlabelled O-linked glycans is shown in the lower of the two chromatograms (B).


Resources
Label-Free Analysis by UHPLC with Charged Aerosol Detection of Glycans Separated by Charge, Size, and Isomeric Structure UHPLC Analysis of 2-Aminobenzamide-Labeled Glycans with the Vanquish Flex UHPLC System Label-free N-Glycan Profiling (Poster) Label-free O-Glycan Profiling (Poster) Label-free N-Glycan Profiling (Poster Brief) Label-free O-Glycan Profiling (Poster Brief) The Sweet Revolution in Glycan and Antibody Separations (Recorded Webinar) Charged Aerosol Detection Applications in Biopharmaceutical Analysis (Recorded Webinar) AppsLab Library - Glycan Methods The Government, Easter Eggs & Biotherapeutics: A Glycosidic Link Detecting the Undetectable
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Thermo Scientific™ GlycanPac™ AXR-1 LC Column Thermo Scientific™ Accucore™ Vanquish™ C18+ UHPLC Column

Charge Variant Analysis

Protein charge homogeneity can have a significant effect on the structure, stability, binding affinity, and efficacy of a biotherapeutic drug. Ion exchange chromatography (IEX) is often used for protein charge variant profiling, either by salt gradient or more recently by pH gradient.

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Antibody variants may have different charge states, meaning separation according to charge is required, usually by IEX.

Our dedicated buffer kits allow set up of pH gradients with ease.

MAbPac SCX-10 is a strong cation exchange column designed specifically for high-resolution separations of antibodies and associated variants by IEX.

Vanquish Flex UHPLC is ideal for routine, high throughput ion exchange chromatography with generic and steep gradients, being fast, robust and reliable and having twice the sample capacity of many UHPLC systems.

Compliant, simple, data management and reporting is performed using Thermo Scientific™ Chromeleon™ CDS.

pH gradient ion exchange of monoclonal antibody (overlay of six runs).

Optimized salt gradient for monitoring deamidation of ribonuclease with ProPac WCX.

Identification and separation of charge variants is performed rapidly and reliably. The use of a pH gradient-based system can be more robust and less challenging than a salt-based system. The unique nonporous pellicular resin used in MAbPac SCX-10 provides high resolving power, permitting the separation of monoclonal antibody variants that differ by as little as one charge.


Resources
Reversed-Phase Separation of Intact Therapeutic Antibodies Using the Vanquish Flex UHPLC System High Salt Gradient Analysis of Post-Translational Modifications - Deamidation Monitoring 'Ultra' Biotherapeutic Characterisation Utilizing the Native Fluorescence of Monoclonal Antibodies for the Sensitive Detection of Charge Variants Transferring a Method for the Characterization of PTMs from the UltiMate 3000 BioRS System to the Vanquish Flex UHPLC System AppsLab Library - Charge Variant Methods
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Thermo Scientific™ pH Buffer Thermo Scientific™ MAbPac™ SCX-10 LC Column

Peptide Mapping

Peptide mapping protocols that detail the entire protein (100% sequence coverage) are required to prove molecular structure as well as determine post-translational modifications (PTMs). Complex protein digests require high peak capacity and high-resolution separations. Our peptide mapping workflow solution includes a fast, reproducible protein digestion protocol and powerful yet simple peptide mapping software, with Vanquish Flex UHPLC at its heart.

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Prior to peptide mapping, proteins must be digested into their constituent peptides, typically using an enzyme.

Thermo Scientific™ SMART Digest™ kits are designed for biopharmaceutical applications that require highly reproducible, sensitive and fast analyses, with digests taking <60 minutes, for high-throughput routine workflows..

UV and MS detection are both widely used in peptide mapping. UV is often used in routine environments and is the workflow described here.

Thermo Scientific™ Acclaim™ VANQUISH™ C18 UHPLC Column is an excellent choice of column, it has an ultrapure silica substrate with extremely low metal content to minimize tailing effects and deliver the sharp, symmetrical peak shapes needed in this workflow.

Vanquish Flex has a very high pressure flow path and sample pressurization prior to injection to ensure high peak capacity, retention time stability and peak area precision, ideal for peptide mapping applications.

MS techniques for intact proteins should ideally be high resolution accurate mass (HRAM) for maximum quantification and structural elucidation.

Total ion chromatograms obtained from peptide mapping experiments of rituximab applying gradient lengths of 30, 20 and 13 minutes.


MS/MS spectrum of the rituximab glycopeptide aa 290-302 (TKPREEQFN*STFR, *=G0) with the typical fragmentation pattern: the two oxonium ions 204 (HexNAc), 366 (Hex-Hex-NAc), and the sequence ladder of the fragmented glycan attached to the intact peptide.

Coupling the Vanquish UHPLC with Orbitrap™ mass spectrometers and Thermo Scientific™ BioPharma Finder™ Software provides accurate, in-depth characterization and relative quantification of biotherapeutic peptides for ultimate peptide mapping confidence.


Resources
Reliable Results in Peptide Mapping Using the Vanquish Flex UHPLC System LC-UV-MS Peptide Mapping Development for Easy Transfer to LC-UV QA/QC High throughput Peptide Mapping by UHPLC-MS (Poster) 'Ultra' Biotherapeutic Characterisation New Integrated Informatics Solution for Protein Biotherapeutics Characterization High throughput Peptide Mapping by UHPLC-MS (Poster Brief) Get Smart with Protein Digestion Improved Characterization of Biotherapeutics with Better Peptide Mapping A Brief History of and Guide to Peptide Mapping Ahoy! Do I Need Satellite Navigation for my Peptide Map? Not All Who Wander Are Lost A Brief History of and Guide to Peptide Mapping AppsLab Library - Peptide Mapping High-Throughput, High-Resolution Peptide Maps
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Thermo Scientific™ SMART Digest™ Kit Thermo Scientific™ Acclaim™ VANQUISH™ C18 UHPLC Column Peptide Mapping Workflows: Fast, confident and more reliable peptide mapping BioPharma Finder Software: Workflow driven informatics for complete biotherapeutic characterization

Intact Mass Analysis

There remains a need to characterize biotherapeutic proteins in an intact form, particularly where there is likelihood of structural information such as isomerism that may not be observed by other techniques. It is also important in some of the novel biotherapeutic classes such as antibody drug conjugates (ADCs). Our range of Thermo Scientific™ MAbPac™ HIC columns are particularly well suited to the characterization of ADCs, allowing DAR determination in minutes.

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mAbs and ADCs require thorough characterization, including glycoforms, higher order structures and PTMs.

MAbPac™ RP is a new reversed phase column that uses a resin based on hydrophobic supermacroporous 4µm polymer particles. It is ideal for the efficient separation of protein molecules with very low carry over.

UHPLC is the most effective technique to ensure separation of higher order structures and unwanted interferents.

MS techniques for intact proteins should ideally be high resolution accurate mass (HRAM) for maximum quantification and structural elucidation.

MS analysis of 100 ng of the mAb Rituximab, depicting (A) LC chromatogram using Thermo Scientific MSPac™ DS-10 Desalter Cartridge, and (B) the deconvoluted spectrum and annotated glycoforms.

The Vanquish Flex UHPLC system readily hyphenates to the full range of Thermo Scientific™ mass spectrometers including gold standard Orbitrap™ mass analyzer instruments. The Thermo Scientific™ Q Exactive BioPharma™ Platform offers a High Mass Range (HMR) mode of operation, ideally suited for the analysis of intact protein complexes, such as mAbs, and ADCs in the native or denatured state.

Reproducibility of protein separation is as important as resolution and sensitivity. Vanquish Flex UHPLC produces excellent separation reproducibility giving you the confidence you need.


Resources
High Salt Gradient Analysis of Post-Translational Modifications - Deamidation Monitoring (Application Brief) SEC-MS with Volatile Buffers for Characterization of Biopharmaceuticals (Application Note) Reversed-Phase Separation of Intact Therapeutic Antibodies Using the Vanquish Flex UHPLC System (Application Note) Fast Online Desalting of mAbs Using a Reversed-Phase Desalting Cartridge for LC-MS Analysis (Application Note) Influence of Column Temperature on Reversed-Phase Chromatography of an Intact Antibody (Technical Note) High Temperature, High Throughput Reversed Phase Separation of Intact Monoclonal Antibodies (Poster Note) Enabling Mass Spectrometric Analysis of Intact Proteins in Native Conditions on A Hybrid Quadrupole-Orbitrap Mass Spectrometer (Poster Note) Full Characterization of Heterogeneous Antibody Samples Under Denaturing and Native Conditions on a Hybrid Quadrupole-Orbitrap Mass Spectrometer (Poster Note) Complete Characterization of a Cysteine-linked Antibody-Drug Conjugate Performed on a Hybrid Quadrupole-Orbitrap Mass Spectrometer with High Mass Range (Poster Note) New Integrated Informatics Solution for Protein Biotherapeutics Characterization (Poster Note) AppsLab Library - Intact Mass Methods
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Thermo Scientific™ MAbPac™ HIC-20 HPLC Column Thermo Scientific™ MAbPac™ RP Column Thermo Scientific™ MSPac™ DS-10 Desalter Cartridge Q Exactive BioPharma: The complete BioPharma characterization solution BioPharma Finder Software: Workflow driven informatics for complete biotherapeutic characterization

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