Vanquish

Product Specifications: Thermo Scientific Vanquish – Variable Wavelength Detector F

Application Brief 71811: High Salt Gradient Analysis of Post-Translational Modifications - Deamidation Monitoring

Technical Note 167: How to Realize LC-MS Quantitation with Chromeleon 7.2 CDS

Application Note 1138: Fast and Ultrafast LC-MS/MS Methods for Robust and Reliable Analysis of Pesticides in Food Using the Vanquish UHPLC System

Application Note 1133: SEC-MS with Volatile Buffers for Characterization of Biopharmaceuticals

Vanquish UHPLC System: Best in UHPLC Column Thermostatting to Fit All Needs

The required technique to control the actual separation temperature is often underestimated and, in many cases, not well understood in UHPLC. Thermostatting has to be effective, but must not compromise the achievable column efficiency. On the other hand, it is very important to account for successful method transfer between different instruments. The Vanquish UHPLC system provides an entirely new and unique combination of active eluent pre-heating and advanced column thermostatting modes.

Application Brief 170: Fast Nevirapine Impurity Profiling Using UHPLC-DAD

Nevirapine is a non-nucleoside reverse transcriptase inhibitor with activity against human immunodeficiency virus type 1 (HIV-1), currently marketed for the treatment of HIV-1 infected adults. Here we report an optimized method using a state of the art gradient UHPLC-UV method. Applying ballistic gradients with latest-generation UHPLC equipment achieves significantly shorter analysis time while maintaining compliance with ICH (International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use) requirements.

Analysis of Subtle Changes in Biological Systems Through use of High Resolution, High Accuracy UHPLC Generated Libraries with a Q-Exactive HF Mass Spectrometer

This poster note describes a robust method for discovering subtle changes in protein expression in biological systems using the new Thermo Scientific Vanquish UHPLC system coupled with the new Thermo Scientific Q-Exactive HF Mass Spectrometer.

How Scalable are the Dispersion Processes in Real Columns Packed with Solid Core Material?

Thermostatting in UHPLC: Forced Air Mode, Still Air Mode, and Method Transfer

This poster note discusses evaluating method portability versus ultimate column efficiency with a new UHPLC platform. The Thermo Scientific Vanquish UHPLC system supports the choice between retention control or efficiency optimization as well as advanced method transfer options.

Technical Note 160: Development of Ultra-fast pH-Gradient Ion Exchange Chromatography for the Separation of Monoclonal Antibody Charge Variants

Column Temperature Control in Peptide Mapping: Maximizing Peak Capacity by Keeping Your Column Hot the Right Way

Application Note 21182: Rapid Screening Method for Polycyclic Aromatic Hydrocarbons Using an Advanced Solid Core UHPLC Column and System Combination

This application note demonstrates the advantages of using the Accucore Vanquish C18+, 1.5 µm column and Vanquish UHPLC system for the fast analysis of polycyclic aromatic hydrocarbons (PAHs). The advanced capabilities of the Vanquish UHPLC system allow the Accucore Vanquish columns to be operated at high flow rates that enable development of rapid analytical methods while maintaining high performance.

Application Note 21183: Rapid Separation of Ibuprofen, Ibuprofen Related Compound C, and Valerophenone Using Advanced UHPLC and Sub-2 µm Solid Core Column Technologies

This application note demonstrates the advantages of using the Accucore Vanquish C18+, 1.5 µm column and Vanquish UHPLC system for the separation of ibuprofen, ibuprofen-related compound C, and valerophenone. The advanced capabilities of the Vanquish UHPLC system allow the Accucore Vanquish UHPLC columns to be operated at higher flow rates that enable development of rapid analytical methods while maintaining performance.

Application Note 1124: Providing the Highest Retention Time and Peak Area Reproducibility for Maximal Confidence in Peptide Mapping Experiments

Stability of retention times and peak areas are crucial for the evaluation of peptide mapping experiments especially in LC-UV type experiments e.g. in process control or stability studies. In this application note, we demonstrate that the Vanquish UHPLC system provided extraordinary stability of retention time and peak area of a very large sample set of 86 injections over more than 80 hours. This stability will enable the analyst to confidently attribute a change in retention time or peak area to a real peptide modification and avoids any misinterpretation of the data.

Application Note 1126: Metoprolol and Select Impurities Analysis

This application note describes a simple, rapid, and accurate method for the analysis of metoprolol succinate and metoprolol EP Impurity A, M and N using isocratic HILIC chromatography with UV and charged aerosol detection that does not require derivatization. The HPLC detectors are orthogonal and complimentary in nature and render a more comprehensive analysis of the sample since they can detect both chromophore and non-chromophore species using the configuration described. The integrated Vanquish system provides excellent data quality.

Application Note 1131: Reversed-Phase Separation of Intact Therapeutic Antibodies Using the Vanquish Flex UHPLC System

In biopharmaceutical early analytical development, characterization of monoclonal antibodies is required to support process development. Separation techniques such as hydrophobic interaction chromatography, size exclusion chromatography, ion-exchange chromatography, and reversed-phase chromatography (RPC) are routinely applied to profile the therapeutic protein during this stage of development. RPC can be run with mass spectrometry (MS)-compatible mobile phase, hence the method can be easily transferred to MS characterization laboratories when required. In this work, the MAbPac-RP column was operated with the Vanquish Flex UHPLC system for the RPC of several intact therapeutic antibodies. To assess the suitability of the approach for stability studies, the separation of a reference and a stressed mAb chromatogram was compared.

Application Note 1132: Reliable Results in Peptide Mapping Using the Vanquish Flex UHPLC System

Peptide mapping is one of the routine methods for biotherapeutics characterization. The Vanquish Flex UHPLC system features a quaternary pump for highest application flexibility. In addition, identically to the Vanquish UHPLC system, the sample is pressurized prior to the injection into the high-pressure flow path. This results in a highly stable flow delivery and thus significantly improves the retention time precision. Thereby the confidence in peak assignment is increased.In this work, the separation of peptides obtained from a bovine serum albumin (BSA) digest is demonstrated. The retention time and peak area precisions are evaluated for repeated injections.

Technical Note 165: Boosting Trace Detection Performance with the Vanquish Diode Array Detector and High-Sensitivity LightPipe Flow Cell

This technical note demonstrates how the Thermo Scientific™ Vanquish™ Diode Array Detector (DAD) HL with a 60 mm high-sensitivity LightPipe™ flow cell boosts UV trace detection performance of HPLC applications. Detection side effects such as refractive index effects and peak dispersion are explained and used to evaluate the data from this flow cell. The peak dispersion data further leads to recommendations which column formats are best suited for this type of flow cell. The Vanquish DAD is available for the Vanquish system platform and the Thermo Scientific™ Dionex™ UltiMate 3000™ UHPLC system platform.

Technical Note 166: Fast and Easy Optimization of Detection Wavelengths with Vanquish Fluorescence Detectors

This technical note describes why the optimal fluorescence wavelength pair can vary between different fluorescence detector models and to demonstrate how users can easily verify and optimize fluorescence detection wavelengths with the Thermo Scientific™ Vanquish™ Fluorescence Detector.