UHPLC always provides better signal to noise than HPLC
Answer:
UHPLC detector technology works with long light paths and low system dispersion to deliver narrow peaks. Ultimate trace detection may be achieved with special flow cells with extended light paths (e.g. of 60 mm length). In addition, up-to-date UHPLC detectors achieve better noise performance than HPLC detectors.
HPLC applications, however, are not as fast as UHPLC separations and can take advantage of longer response times. These longer response times smooth the baseline, to-noise ratio. Overall, the application parameters dictate which of these factors will dominate the signal to noise performance.
One thing is for sure: Whether you are running HPLC or UHPLC applications, the latest detector technology, such as the Vanquish Diode Array Detector, will provide you with the best sensitivity.
Peer Quiz Results:
Fact — UHPLC systems have less dispersion and therefore provide higher peaks
Demanding UHPLC methods always come with poor reproducibility.
Answer:
Precision of the retention time (RT) is measured in % RSD. This parameter typically gets higher when runtime is reduced on the same instrument. This means that UHPLC equipment needs to improve all the parameters that influence the retention time reproducibility to keep up with the requirements of fast analysis.
The improved flow precision, gradient formation and temperature control on the Vanquish system result in retention time precision previously unachieved by HPLC or UHPLC.
Peer Quiz Results:
Fact — This is what I experience as well
No one needs UHPLC. Solid Core columns in an HPLC system are good enough.
Answer:
Solid core column technology has made remarkable progress, as have UHPLC pressure capabilities. Each of the individual technologies is great, but we think the best approach is to combine them. This is exactly what we did with the Accucore Vanquish solid core sub-2 µm columns. Just like the Vanquish UHPLC system, these columns can operate at pressures up to 1500 bar (22,000 psi).
The combination of system and column provides state of the art chromatography, as the lowest dispersion of the Vanquish system is ideal to conserve the efficiency provided by the Accucore Vanquish columns, until your data is entered into your report. Learn more about the brand new Accucore Vanquish column capabilities.
Peer Quiz Results:
Solid core particles with my HPLC system are the best combination.
More UHPLC throughput does not help, data analysis and reporting are the main bottleneck
It's fairly common to find empty labs full of UHPLC systems. Often, operators are behind their desks processing results, a consequence of lengthy data processing. This is where Chromeleon Chromatography Data System comes in. In addition to controlling the Vanquish system and setting up the experiments with ease, it also processes results. The beauty of Chromeleon CDS is that it uses a familiar Excel-based spreadsheet for the results and provides dynamic reprocessing. Should you need to make an adjustment to the data analysis, that adjustment is automatically applied to all relevant runs, saving huge amounts of time, and opening the door for even more throughput.
Peer Quiz Results:
Fact — I spend more time on reviewing the data, than actually acquiring it
UHPLC is all about performance, not ease of use
Answer:
We think that there should be no difference between operating HPLC and UHPLC instruments. Therefore, in 2010 we adopted a UHPLC-focused philosophy: to make UHPLC available for all users, in all laboratories, for all analytes. We believe that the extra performance of UHPLC should not come with more complicated operation. Thus, our goal with the new Vanquish system is to simultaneously enhance performance and ease-of-use. Interaction with the system is easy thanks to tool-free maintenance parts, automated routine setup features, Viper fingertight connections, and, of course, the gold standard in chromatography software, Chromeleon Chromatography Data System.
Peer Quiz Results:
Fact — True for many UHPLC systems
UHPLC systems are so fast, they run out of samples over a weekend.
Answer:
There are 3840 minutes in a weekend and, with fast run times, several thousand samples can be analyzed during that time. The Vanquish system already has a sample capacity of 4 sample racks, which is twice the industry average. Combined with the Charger module, the Vanquish system can manage up to 23 well plates. With 2208 samples for 96 well plates and 8832 samples for 384 well plates, that is more than enough to keep the system running through the entire weekend.
UHPLC uses less sample, causing inferior area precision.
Answer:
Injection precision could suffer from smaller UHPLC injection volumes because the absolute error has a larger relative contribution. This means that the absolute error should be minimized, something that the Vanquish system takes into account. All relevant parts of the injection process are optimized for the best precision and for excellent reproducibility, even at 1 µL or less. At the same time, the Vanquish system supports injection volumes up to 25 µL. With an optional sample loop, the injection volume can be increased to 100 µL.
No matter what your sample size is, the Vanquish system will always perform excellent and reliable injections.
The mass information in MS reduces the need for retention time reproducibility.
Answer:
Mass spectrometry data gives an additional dimension of information, which can differentiate analytes in the case of co-eluting compounds. When quantitative information is desired, mass spectrometry will actually benefit from improved separations and excellent retention time reproducibility. The improved separation will minimize co-elution and ion suppression effects maximizing the signal. In addition, the retention time stability allows scheduling narrower transition windows in SRM type experiments, which increase the amount of information obtained. The Vanquish system's improved separations increase the data that can be obtained from an LC-MS experiment.
UHPLC fluidics are not biocompatible and difficult to connect.
Answer:
It is true that UHPLC connections need to withstand higher pressures, something not all biocompatible materials are capable of. But with the Viper fingertight fitting system, we have revolutionized the way connections are made in UHPLC. The special construction allows tool-free operation, while robustly sealing at pressures up to 1500 bar. Viper capillaries are also available in biocompatible format, and these come standard for the Vanquish system. This ensures that UHPLC is not only set up with ease, but also biocompatible and applicable to analyze your biologics, from proteins to peptides or DNA.
The column heating technology does not impact the LC method transfer.
Answer:
Hosting the LC column means so much more than simply "hold and heat." Pressure and viscosity lead to frictional heat inside the column. This can interfere with the chromatography if not handled adequately.
The Vanquish column compartment offers two thermostatting modes, in addition to hybrid versions, to match all application needs. Ensure the best efficiency by keeping the frictional heat within the column with the so called "still-air" mode. Improve method transfer from other HPLC or UHPLC systems by tuning constant separation temperature through the removal of frictional heat.
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